10、e increases and decreases in conductance fitted by a step-finding algorithm (red), showing the zero- to five-step growing and declining processes. b) Histograms of the step numbers in growing (above) and declining (bottom) data counted from thousands of current oscillating events. c) The kinetic zip 。

11、per model for the unfolding and folding processes of a hairpin DNA with five base pairs in stem. de) Representative five-step increasing and decreasing data of Device 3,荧光相关光谱 - 先进共聚焦荧光显微镜 E.coli bacterium(approx.0.2fL,G(0): the autocorrelation function at time 0 1 : the chacteristic diffusion time。

12、of the particle though the sample volume. P: is the structure parameter, which is the ratio between the transversal and longitudinal radius of the sample volume p=1/ 2,荧光强度可以用自相关方程(G(t)来分析,荧光相关光谱,荧光相关光谱 - 自相关曲线(auto-correlation curve,荧光相关光谱-浓度测量 (Eigen, PNAS, 1994, 5740;
Schwille, Biophys.J. 1997,187 。

13、8,TheVeff can be easily obtained from calibration experiment, and G(0) can be read out from the auto-correlation curve,Studies on the formation and stability of triplexDNA using fluorescence correlation spectroscopy,Luminescence 2016;
31: 830836,A simulation-guided fluorescence correlation spectrosc 。

14、opy tool to investigate the protonation dynamics of cytochrome c oxidase,Scheme of CcOs proton (red) and electron (blue) pathways in the structural model of CcO (PDB: 3HB3). Coppers CuA/CuB (orange spheres) and hemes a/a3 are indicated. Top view onto the cytoplasmic surface,Schematic of the experime 。

15、ntal FCS setup. (b) the fluctuating fluorescence intensity over time.(c) The pH titration curve for 5-IAF. d) the autocorrelation is calculated and exhibits distinctive decay components with characteristic times t for D, p,app and t,app,PCCP., 2016, 18, 12877,PCCP., 2016, 18, 12877,Autocorrelation c 。

16、urves of fluorescein in 0.1 M NaOH at three different laser intensities (1 mW, 2 mW, 20 mW). The power was reduced from (a) to (c,Schematic outline of the single-molecule based simulation of fluorescence correlation spectroscopy data,PCCP., 2016, 18, 12877,Protonation rate kp,app as a function of pr 。

17、oton concentration for CcO CS-K299C-AF,Studies of cytochrome c-551 unfolding usingfluorescence correlation spectroscopy and other biophysical techniques,Backbone representation of the crystal structure of horse heart cytc (left, Pdb id:1HRC) and cytc551 (right, Pdb id:2PAC,Pictorial representation o 。

18、f the overall shape change of cytc551 during urea induced unfolding transition. N, I and U in the figure represent the native, intermediate and unfolded state of cytc551,PCCP., 2016, 18, 24537,PCCP., 2016, 18, 24537,Correlation functions obtained with Q46TMR in the folded state. (a) generate a non-r 。

19、andom residual distribution .( b)The correlation function of Q46TMR was also fit using the one diffusion one exponential model. (c)The addition of a second exponential component did not improve the fit any further. (d)variation of rH of the globular protein in the native state with amino acid residues;
(e)The steady state fluorescence spectra of Q46TMR, K33TMR and V5TMR,单分子检测的应用,超灵敏分析和仪器微型化 .活细胞分析 .DNA测序 .分子马达 .单分子动力学 。

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标题：现代光学分析第9章|现代光学分析：第9章 单分子分析( 二 )